vectashilde r○ mounting medium Search Results


medium with dapi  (Novus Biologicals)
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Novus Biologicals medium with dapi
Medium With Dapi, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectashield mounting medium with dapi  (Vector Laboratories)
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Vector Laboratories vectashield mounting medium with dapi
( A ) Nedd4 KD increases mHtt aggregation in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Cells were fixed and immunostained for Htt followed by confocal microscopy. <t>DAPI</t> was included in mounting medium to stain nuclei. ( B ) Nedd4 KD increases the size and the number of mHtt aggregates in primary cortical neurons. n = 5 microscopic fields for each, 2-tailed unpaired t test (*** P < 0.001). ( C ) Nedd4 KD enhances Htt toxicity in an mHtt expression-dependent manner in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Pictures were taken with bright-field microscope to show cell morphology.
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( A ) Nedd4 KD increases mHtt aggregation in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Cells were fixed and immunostained for Htt followed by confocal microscopy. <t>DAPI</t> was included in mounting medium to stain nuclei. ( B ) Nedd4 KD increases the size and the number of mHtt aggregates in primary cortical neurons. n = 5 microscopic fields for each, 2-tailed unpaired t test (*** P < 0.001). ( C ) Nedd4 KD enhances Htt toxicity in an mHtt expression-dependent manner in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Pictures were taken with bright-field microscope to show cell morphology.
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vectashield r  (Vector Laboratories)
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Vector Laboratories vectashield r
( A ) Nedd4 KD increases mHtt aggregation in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Cells were fixed and immunostained for Htt followed by confocal microscopy. <t>DAPI</t> was included in mounting medium to stain nuclei. ( B ) Nedd4 KD increases the size and the number of mHtt aggregates in primary cortical neurons. n = 5 microscopic fields for each, 2-tailed unpaired t test (*** P < 0.001). ( C ) Nedd4 KD enhances Htt toxicity in an mHtt expression-dependent manner in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Pictures were taken with bright-field microscope to show cell morphology.
Vectashield R, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Nedd4 KD increases mHtt aggregation in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Cells were fixed and immunostained for Htt followed by confocal microscopy. <t>DAPI</t> was included in mounting medium to stain nuclei. ( B ) Nedd4 KD increases the size and the number of mHtt aggregates in primary cortical neurons. n = 5 microscopic fields for each, 2-tailed unpaired t test (*** P < 0.001). ( C ) Nedd4 KD enhances Htt toxicity in an mHtt expression-dependent manner in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Pictures were taken with bright-field microscope to show cell morphology.
Vectashield(r) Antifade Mounting Medium, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectashield r antifade mounting medium  (Vector Laboratories)
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Vector Laboratories vectashield r antifade mounting medium
( A ) Nedd4 KD increases mHtt aggregation in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Cells were fixed and immunostained for Htt followed by confocal microscopy. <t>DAPI</t> was included in mounting medium to stain nuclei. ( B ) Nedd4 KD increases the size and the number of mHtt aggregates in primary cortical neurons. n = 5 microscopic fields for each, 2-tailed unpaired t test (*** P < 0.001). ( C ) Nedd4 KD enhances Htt toxicity in an mHtt expression-dependent manner in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Pictures were taken with bright-field microscope to show cell morphology.
Vectashield R Antifade Mounting Medium, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Nedd4 KD increases mHtt aggregation in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Cells were fixed and immunostained for Htt followed by confocal microscopy. <t>DAPI</t> was included in mounting medium to stain nuclei. ( B ) Nedd4 KD increases the size and the number of mHtt aggregates in primary cortical neurons. n = 5 microscopic fields for each, 2-tailed unpaired t test (*** P < 0.001). ( C ) Nedd4 KD enhances Htt toxicity in an mHtt expression-dependent manner in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Pictures were taken with bright-field microscope to show cell morphology.
Vectashield(r) Antifade Mounting Medium With Dapi, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectashield antifade mounting medium  (Novus Biologicals)
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( A ) Nedd4 KD increases mHtt aggregation in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Cells were fixed and immunostained for Htt followed by confocal microscopy. <t>DAPI</t> was included in mounting medium to stain nuclei. ( B ) Nedd4 KD increases the size and the number of mHtt aggregates in primary cortical neurons. n = 5 microscopic fields for each, 2-tailed unpaired t test (*** P < 0.001). ( C ) Nedd4 KD enhances Htt toxicity in an mHtt expression-dependent manner in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Pictures were taken with bright-field microscope to show cell morphology.
Vectashield Antifade Mounting Medium, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectashield r© hardsettm mounting medium  (BioLynx Inc)
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( A ) Nedd4 KD increases mHtt aggregation in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Cells were fixed and immunostained for Htt followed by confocal microscopy. <t>DAPI</t> was included in mounting medium to stain nuclei. ( B ) Nedd4 KD increases the size and the number of mHtt aggregates in primary cortical neurons. n = 5 microscopic fields for each, 2-tailed unpaired t test (*** P < 0.001). ( C ) Nedd4 KD enhances Htt toxicity in an mHtt expression-dependent manner in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Pictures were taken with bright-field microscope to show cell morphology.
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vectashield mounting medium  (Novus Biologicals)
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Novus Biologicals vectashield mounting medium
( A ) Nedd4 KD increases mHtt aggregation in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Cells were fixed and immunostained for Htt followed by confocal microscopy. <t>DAPI</t> was included in mounting medium to stain nuclei. ( B ) Nedd4 KD increases the size and the number of mHtt aggregates in primary cortical neurons. n = 5 microscopic fields for each, 2-tailed unpaired t test (*** P < 0.001). ( C ) Nedd4 KD enhances Htt toxicity in an mHtt expression-dependent manner in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Pictures were taken with bright-field microscope to show cell morphology.
Vectashield Mounting Medium, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectar shield vibrance antifade mounting medium with dapi  (Vector Laboratories)
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( A ) Nedd4 KD increases mHtt aggregation in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Cells were fixed and immunostained for Htt followed by confocal microscopy. <t>DAPI</t> was included in mounting medium to stain nuclei. ( B ) Nedd4 KD increases the size and the number of mHtt aggregates in primary cortical neurons. n = 5 microscopic fields for each, 2-tailed unpaired t test (*** P < 0.001). ( C ) Nedd4 KD enhances Htt toxicity in an mHtt expression-dependent manner in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Pictures were taken with bright-field microscope to show cell morphology.
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( A ) Nedd4 KD increases mHtt aggregation in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Cells were fixed and immunostained for Htt followed by confocal microscopy. <t>DAPI</t> was included in mounting medium to stain nuclei. ( B ) Nedd4 KD increases the size and the number of mHtt aggregates in primary cortical neurons. n = 5 microscopic fields for each, 2-tailed unpaired t test (*** P < 0.001). ( C ) Nedd4 KD enhances Htt toxicity in an mHtt expression-dependent manner in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Pictures were taken with bright-field microscope to show cell morphology.
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Image Search Results


( A ) Nedd4 KD increases mHtt aggregation in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Cells were fixed and immunostained for Htt followed by confocal microscopy. DAPI was included in mounting medium to stain nuclei. ( B ) Nedd4 KD increases the size and the number of mHtt aggregates in primary cortical neurons. n = 5 microscopic fields for each, 2-tailed unpaired t test (*** P < 0.001). ( C ) Nedd4 KD enhances Htt toxicity in an mHtt expression-dependent manner in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Pictures were taken with bright-field microscope to show cell morphology.

Journal: JCI Insight

Article Title: Ubiquitin ligase Nedd4 regulates the abundance and toxicity of mutant huntingtin

doi: 10.1172/jci.insight.181013

Figure Lengend Snippet: ( A ) Nedd4 KD increases mHtt aggregation in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Cells were fixed and immunostained for Htt followed by confocal microscopy. DAPI was included in mounting medium to stain nuclei. ( B ) Nedd4 KD increases the size and the number of mHtt aggregates in primary cortical neurons. n = 5 microscopic fields for each, 2-tailed unpaired t test (*** P < 0.001). ( C ) Nedd4 KD enhances Htt toxicity in an mHtt expression-dependent manner in primary cortical neurons. Mouse primary cortical neurons were transduced with lentivirus expressing Htt Exon1-25Q or -72Q together with scrambled (scr) or shNedd4-35 (N4-35). Pictures were taken with bright-field microscope to show cell morphology.

Article Snippet: The following reagents were purchased as indicated: DMEM (11995065, Thermo Fisher), L-Glutamine (25030081, Thermo Fisher), Penicillin-Streptomycin (15140122, Thermo Fisher), Neurobasal media (21103049, Thermo Fisher), B-27 Supplement (17504044, Thermo Fisher), N-ethylmaleimde (23030, Thermo Fisher), PMSF (36978, Thermo Fisher), ZeptoMetrix RETROtek HIV-1 p24 Antigen ELISA (22-156-700, Fisher), FBS (100-106, Gemini Bio), CHX (14126, Cayman Chemical), bafilomycin A1 (11038, Cayman Chemical), bortezomib (#2204, Cell Signaling Technology), Ponseau S (P7170, Sigma), rapamycin (R8781, Sigma), cOmplete mini EDTA-free protease inhibitor cocktail (11836170001, Sigma), Protein A-Sepharose (P3391, Sigma), Protein G-Agarose (P4691, Sigma), Vectashield mounting medium with DAPI (H-1200, Vector Laboratories), Lenti-X concentrator (631232, TaKaRa), RNase-free DNase (79254, Qiagen), and Ub aldehyde (U-201-050, R&D Systems).

Techniques: Transduction, Expressing, Confocal Microscopy, Staining, Microscopy